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Volume 7, issue 2 | Copyright

Special issue: Dresden Sensor Symposium 2017

J. Sens. Sens. Syst., 7, 433-441, 2018
https://doi.org/10.5194/jsss-7-433-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Regular research article 06 Jul 2018

Regular research article | 06 Jul 2018

Metal ion binding and tolerance of bacteria cells in view of sensor applications

Jonas Jung1, Anja Blüher1, Mathias Lakatos2, and Gianaurelio Cuniberti1 Jonas Jung et al.
  • 1Institute for Materials Science and Max Bergmann Center of Biomaterials, TU Dresden, 01062 Dresden, Germany
  • 2Chair of Physical Chemistry, Measurement and Sensor Technology, TU Dresden, 01062 Dresden, Germany

Abstract. The biotechnological use of bacterial cells and cell components for the detection and accumulation of valuable substances, such as metals and rare earth elements in aqueous systems, is possible by utilizing innate binding characteristics of microorganisms. We have studied the bacteria cells of Lysinibacillus sphaericus JG-B53 and Sporosarcina ureae ATCC 13881 to assess their potential applicability for the detection of rare earth elements, base metals or precious metals in water. First, we have demonstrated the interactions of the cells with the metal complexes of Au, Ho and Y by studying the color changes of the respective solutions, scanning electron microscopy (SEM) imaging of the metal cluster decoration on the cell surfaces and cell growth tolerance tests. Based on these results, we have developed two potential sensor systems. A colorimetric sensor was established by applying gold nanoparticles (AuNPs) functionalized with surface-layer (S-layer) proteins SslA of S. ureae ATCC 13881 or Slp1 of L. sphaericus JG-B53 for the selective detection of YCl3 up to 1.67 × 10−5molL−1 in water. Additionally, a regenerative sensor layer of S-layer proteins on a thin gold film was developed for the detection of 1 × 10−4molL−1 YCl3 in water by surface plasmon resonance (SPR) spectroscopy.

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We assessed the applicability of bacterial surface layer proteins of Lysinibacillus sphaericus JG-B53 and Sporosarcina ureae ATCC 13881 for the detection of metal ions in water. Based on the interactions of the cell components with metal complexes, two potential sensor systems, one colorimetric with functionalized gold nanoparticles and the other using a regenerative sensor layer, were developed. The systems' detection limits of YCl3 in water were 1.67 x 10−5 and 1 x 10−4 mol L−1, respectively.
We assessed the applicability of bacterial surface layer proteins of Lysinibacillus sphaericus...
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