Journal cover Journal topic
Journal of Sensors and Sensor Systems An open-access peer-reviewed journal
J. Sens. Sens. Syst., 6, 259-267, 2017
https://doi.org/10.5194/jsss-6-259-2017
© Author(s) 2017. This work is distributed under
the Creative Commons Attribution 3.0 License.
Regular research article
27 Jul 2017
A DNA biosensor for molecular diagnosis of Aeromonas hydrophila using zinc sulfide nanospheres
Masoud Negahdary1,2, Mahnaz Jafarzadeh3, Roya Rahimzadeh4, Ghasem Rahimi1, and Hamideh Dehghani2 1Young Researchers and Elite Club, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran
2Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3Young Researchers and Elite Club, Urmia Branch, Islamic Azad University, Urmia, Iran
4Department of Biology, Payame Noor University, Tehran, Iran
Abstract. Today, identification of pathogenic bacteria using modern and accurate methods is inevitable. Integration in electrochemical measurements with nanotechnology has led to the design of efficient and sensitive DNA biosensors against bacterial agents. Here, efforts were made to detect Aeromonas hydrophila using aptamers as probes and zinc sulfide (ZnS) nanospheres as signal enhancers and electron transfer facilitators. After modification of the working electrode area (in a screen-printed electrode) with ZnS nanospheres through electrodeposition, the coated surface of a modified electrode with ZnS nanospheres was investigated through scanning electron microscopy (SEM). The size of synthesized ZnS nanospheres was estimated at about 20–50 nm and their shape was in the form of porous plates in microscopic observations. All electrochemical measurements were performed using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and constant potential amperometry (CPA) techniques. The designed DNA biosensor was able to detect deoxyribonucleic acid (DNA) of Aeromonas hydrophila in the range 1.0  ×  10−4 to 1.0  ×  10−9 mol L−1; the limit of detection (LOD) in this study was 1  ×  10−13 mol L−1. This DNA biosensor showed satisfactory thermal and pH stability. Reproducibility for this DNA biosensor was measured and the relative standard deviation (RSD) of the performance of this DNA biosensor was calculated as 5 % during 42 days.

Citation: Negahdary, M., Jafarzadeh, M., Rahimzadeh, R., Rahimi, G., and Dehghani, H.: A DNA biosensor for molecular diagnosis of Aeromonas hydrophila using zinc sulfide nanospheres, J. Sens. Sens. Syst., 6, 259-267, https://doi.org/10.5194/jsss-6-259-2017, 2017.

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